Folia Medica Indonesiana
ISSN 0303-7932
Vol. 46 / No. 3 / Published : 2010-07
Order : 3, and page :172 - 178
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Original Article :
Antimicrobial activity of mahkota dewa [phaleria macrocarpa (scheff). boerl.] leaf extract against pseudomonas aeruginosa by agar dilution and scanning electron microscopy
Author :
- Muhammad Ali Shodikin*1
- Department of Medical Microbiology Faculty of Medicine, Jember University
Abstract :
Pseudomonas aeruginosa can develop multiple resistances to some antimicrobial agents. The research conducted to found new antimicrobial substances from natural product which cheap, effective and safe. Mahkota dewa (Phaleria macrocarpa) are plants originally grown in Papua, Indonesia. Phytochemical screening of Phaleria macrocarpa leaf showed the existence of flavonoids, polyphenols, saponins and tannins. These substances from another plant are known to have antimicrobial activity.oweverHow However, the antimicrobial activity of Phaleria macrocarpa leaf extract against P. aeruginosa has not been known. The aim of this research was to determine the antimicrobial activity, the minimum inhibitory concentration (MIC) and the effect of antimicrobial activity of Phaleria macrocarpa leaf extract to the P. aeruginosa cells. This experiment used the post test only control group design. Three steps research were conducted, the first was ethanol extraction of Phaleria macrocarpa leaf by maceration. The second was agar dilution antimicrobial testing against P. aeruginosa ATCC 27853. The final step was Scanning Electron Microscopy (SEM) observation. P. aeruginosa were grew on media which contain Phaleria macrocarpa leaf extract on concentration 0-3 x 104 µg/ml, but not grew on concentration 4 x 104 µg/ml and 5 x 104 µg/ml for all replication, therefore the MIC was 4 x 104 µg/ml. The SEM observation demonstrated division inhibition and elongation of P. aeruginosa’s cell. In conclusion, we found the MIC of Phaleria macrocarpa leaf extract against P. aeruginosa was 4 x 104µg/ml. It was very high and reflected weak antimicrobial potency, so it is not suggested to use it as antimicrobial agent against P. aeruginosa.
Keyword :
Antimicrobial activity, MIC, Phaleria macrocarpa leaf, Pseudomonas aeruginosa, SEM,
References :
Boyd RF,(1995) Basic Medical Microbiology. 5th ed Boston : Little Brown and Company
Brooks GF, Butel JS, Ornston LN,(2007) Jawetz, Melnick & Adelberg Medical Microbiology, 24th ed - : McGraw-Hill Companies
Dey PM, and Harborne JB,(1991) Methods in Plant Biochemistry, Volume 6 Assay for Bioactivity London : Academic Press
Archive Article
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 Volume : 46 / No. : 3 / Pub. : 2010-07 |
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